The lab studies how the cytoskeleton is remodeled during collective cell migration, and how these same mechanisms are subverted during cancer invasion. Work in this line uses function-separation mutants and a multi-scale approach — from reconstituted proteins to cellular and in vivo models — combining biochemistry, single-molecule assays, genetics, proteomics, cell biology, live quantitative imaging and super-resolution microscopy.

In-house live-cell imaging (a Leica DMi8 microscope, with up to four-colour multipoint fixed and live imaging) supports this work, including traction-force measurements and immunofluorescence.

Actin cytoskeleton network, confocal microscopy — Juanes Lab